raav2 retro hsyn mcherry  (Addgene inc)

Journal: bioRxiv

Article Title: Spatial and temporal locomotor learning in mouse cerebellum

doi: 10.1101/389965

Figure Lengend Snippet: A) Experimental protocol for assessing lateralization of split-belt learning. Animals with unilateral cerebellar injections underwent adaptation protocols where the fast belt was either ipsilateral (ipsi-fast, right) or contralateral (contra-fast, left) to the cerebellar injection site. Mice in each condition were run first with intra-peritoneal of saline, and subsequently with intra-peritoneal injections of CNO. B) Example cerebellar section from a mouse with unilateral injection of retrograde DREADDs in the interposed nucleus. The borders of the interposed nucleus (ip) are indicated by a white, dashed line. Sample was stained with DAPI (blue) and anti-mCherry (for DREADD expression). C) Average front limb center of oscillation (spatial) adaptation curves (± SEM) for ipsi-fast (left, pink) and contra-fast (right, purple) conditions. In each plot, black curves represent mice under saline conditions; colors represent CNO. D) Average center of oscillation after-effect magnitudes (first 2 washout trials) for ipsi- and contra-fast conditions, with saline and CNO. Individual animals are shown with thin, dashed lines. E) Average double support (temporal) adaptation curves (± SEM) for ipsi- and contra-fast conditions, plotted as in C. F) Average center of oscillation aftereffects (first 2 washout trials) for ipsi- and contra-fast conditions, with saline and CNO. Significance was assessed with paired t -tests.

Article Snippet: We injected retrograde, double floxed Gi-coupled hM4D DREADD (rAAV2 retro-hSyn-DIO-hM4D(Gi)-mCherry; addgene plasmid #44362, ) into the deep cerebellar nuclei of L7-cre animals to target Purkinje cells terminating in each nucleus.

Techniques: Injection, Staining, Expressing